Since colony growth parameters (e.g. colony linear radial growth rate) depend on strain characteristic properties and agar medium composition/physical properties, reference growth parameters need to be measured with a control dataset for comparative analyses.
In the Main tab, upon clicking on the button Define reference growth parameters, the user can define the reference radial growth rate and appearance time either by providing known values manually, or by directing ColTapp to a folder containing a control experiment monitored by time-lapse and already analyzed. In the latter case, the average colony reference appearance time and radial growth rate from that experiment will be automatically assigned. (See Options regarding the averaging method used)
The reference radial growth rate may be used for apperance time estimation from endpoint images. The reference appearance time can be used for calibration of the raw appearance time in given experimental conditions.
